Electrophoresis
PrimoScience Labs (PSL) offer various modes of electrophoresis including SDS-PAGE, IEF, 2D gel separation, and Western blotting.
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
SDS-PAGE is a method used to separate and analyze proteins based on size. This procedure can be used to determine:
- Product purity
- Product identity
- Impurities
- Product stability
Isoelectric Focusing (IEF)
Isoelectric focusing (IEF) separates proteins and peptides based on their isoelectric points (pIs). The method can be used for assessing:
- Protein charge heterogeneity
- Product identity
- Impurities
- Product stability
2-D Gel Electrophoresis
2-D gel electrophoresis is a technique used for separating proteins based on charge and size; its use is primarily for separating protein species in complex mixtures.
Western Blot
Western blot is a technique employed for separating, detecting, and identifying specific proteins in complex mixtures using antibodies specific to the target proteins of interest. The method is used to detect:
- Impurities
- Protein identification
- Protein expression
- Post-translational modifications