Spectroscopy/Spectrometry
PSL provides mass spectrometry services in support of pK studies and product analysis, along with UV-Vis and fluorescence spectroscopy for product characterization.
Ultraviolet-visible (UV-Vis) Spectroscopy
Ultraviolet-visible (UV-Vis) spectroscopy is an analytical method that allows for measurement of light absorption by a sample. The procedure can be used for:
- Identity of small molecules with unique absorption bands
- Purity assessment for small molecules
- Quantification of nucleotide concentration at 260nm
- Quantification of protein concentration at 280nm
Fluorescence Spectroscopy
Fluorescence spectroscopy is an electromagnetic methodology that allows for measurement of fluorescence emission from a sample. It can be used to evaluate:
- Structural environment of tryptophan residues in proteins
- Differentiate between native and unfolded proteins
- In combination with accurate temperature modulation, the transition temperature (Tm) of a protein from native to denatured state
Mass Spectrometry
Mass spectrometry is an analytical procedure used to measure mass to charge ratio in one or more molecules in a sample. The method is useful for:
- Accurate mass analysis of small and large molecules
- Structural analysis of small and large molecules
- Quantitation of metabolites
- Quantitation of small molecules in complex matrices
- Quantitation of pesticides